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Effect of stocking density on the growth and survival of Anomalocardia brasiliana (Gmelin, 1791) (Bivalvia: Veneridae) post-larvae Ciência Rural
Oliveira,Isabela Bacalhau de; Lavander,Henrique David; Lima,Priscilla Celes Maciel de; Oliveira,Carlos Yure Barbosa de; Dantas,Danielli Matias de Macedo; Gálvez,Alfredo Olivera.
ABSTRACT: Anomalocardia brasiliana post-larvae were cultured for 28 days to evaluate the effect of stocking density on their growth and survival rate. Three stocking densities were tested 40, 80 and 160 post-larvae cm-2, in experimental units (2-L) with static cultivation system and total water renewal at each 48 hours. The experimental design was completely randomized with three treatments and three repetitions each. Stocking density of 40 post-larvae cm-2 reached 1 mm length within 24 days, while only 18% of the post-larvae at density of 80 post-larvae cm-2 reached 1 mm length in the same time. The density of 40 post-larvae cm-2 had the highest specific growth rate, 4.98±0.08% day-1. The survival rates of post-larvae at lower densities were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture; Clams; Larviculture; Microalgae; West Indian pointed venus.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200652
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Effects of nitrogen limitation on Dunaliella sp.–Alteromonas sp. interactions: from mutualistic to competitive relationships. ArchiMer
Le Chevanton, Myriam; Garnier, Matthieu; Lukomska, Ewa; Schreiber, Nathalie; Cadoret, Jean-paul; Saint-jean, Bruno; Bougaran, Gael.
Interactions between photosynthetic and non-photosynthetic microorganisms play an essential role in natural aquatic environments and the contribution of bacteria and microalgae to the nitrogen cycle can lead to both competitive and mutualistic relationships. Nitrogen is considered to be, with phosphorus and iron, one of the main limiting nutrients for primary production in the oceans and its availability experiences large temporal and geographical variations. For these reasons, it is important to understand how competitive and mutualistic interactions between photosynthetic and heterotrophic microorganisms are impacted by nitrogen limitation. In a previous study performed in batch cultures, the addition of a selected bacterial strain of Alteromonas sp....
Tipo: Text Palavras-chave: Interactions; Nitrogen limitation; Microalgae; Bacteria; Chemostat; Dissolved organic matter (DOM); Dunaliella.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00344/45529/45087.pdf
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Enhanced single-cell oil production by cold shock in cyanobacterial cultures Ciência Rural
Queiroz,Maria Isabel; Maroneze,Mariana Manzoni; Manetti,Adriana Gonçalves da Silva; Vieira,Juliana Guerra; Zepka,Leila Queiroz; Jacob-Lopes,Eduardo.
ABSTRACT: The aim of this research was to evaluate the enhanced single-cell oil production by cold shock in Aphanothece microscopica Nägeli using dairy processing wastewater as culture medium. The study focused on (i) temperature optimization for biomass production, (ii) cold shock application to induce lipids biosynthesis and (iii) determination of fatty acids profile under different conditions. Results indicated that temperature of 20°C was the best condition in terms of kinetics parameter, reaching biomass productivities of 160.25mg/L.h. Under these conditions, a lipid content of 12.65% was also observed, resulting in a lipid productivity of 20.27mg/L.h. Additionally, the 0°C cold shock was the most efficient in increasing intracellular lipid content,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aphanothece microscopica Nägeli; Dairy wastewater; Lipid; Microalgae; Temperature stress.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100750
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Enhancement of pigment extraction from B. braunii pretreated using CO2 rapid depressurization BJM
Uquiche,Edgar; Antilaf,Ivette; Millao,Sonia.
Abstract Extraction of compounds from microalgae requires cell disruption as a pretreatment to increase extraction yield. Botryococcus braunii is a microalga with a significant content of carotenoids and other antioxidant compounds, such as chlorophylls. Cell disruption of B. braunii using CO2 rapid depressurization was studied as a pretreatment for the extraction of carotenoid and chlorophyll pigments. We studied the effect of temperature (21–49 °C) and pressure (6–13 MPa) during static compression on pigment recovery with supercritical CO2 at 40 °C, 30 MPa and solvent flow of 4.7 L NPT/min. Within the experimental region, the extraction yield of carotenoids and chlorophylls increased by 2.4- and 2.2-fold respectively. Static compression conditions of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Botryococcus braunii; Cell disruption; Chlorophylls; Microalgae; Supercritical extraction.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200497
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Estudo prospectivo de tecnologias baseadas na biomassa de microalgas e cianobactérias: abordagens bibliométrica e patentométrica. Infoteca-e
SCHULTZ, E. L.; QUINTINO, R. P.; ANJOS, S. S. N. dos.
A Embrapa Agroenergia possui seis Linhas de Atuação, as quais correspondem às seis plataformas industriais de biorrefinarias: (i) açúcares C5/C6, (ii) óleos vegetais, (iii) biogás, (iv) algas, v) lignina e vi) bio-óleo/syngas. O objetivo geral deste estudo é identificar, por técnicas de patentometria e bibliometria, as tecnologias baseadas no uso da biomassa de microalgas e cianobactérias, no contexto mundial. Os resultados obtidos geraram diversas informações de cunho estratégico, como os principais processos que utilizam microalgas e cianobactérias, bem como os produtos obtidos e os gêneros taxonômicos usados. Além disso, os principais campos tecnológicos encontrados em documentos de patente foram mostrados e analisados, incluindo o mapa de emergência e...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Bibliometria; Cianobactéria; Patentometria; Estudo prospectivo; Microalga; Patente; Pesquisa; Biomassa; Microalgae; Cyanobacteria; Bibliometric analysis; Patents; Biomass.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1134493
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Etude de nouvelles cibles moleculaires de cancer bronchopulmonaire non a petites cellules pharmacomodulées par des substances originales naturelles et synthetiques ArchiMer
Moreau, Dimitri.
Still rare disease at the beginning of the 20th century, the lung cancer is at the origin of more than 6 % of the deaths in France currently. It occupies there the first rank of the deaths by cancer whereas France is regarded as a country at the average risk among the Western countries. The discovery of new anti-cancer treatments increasingly more effective and specific, is thus today of primary importance. The validation of the capacity of a molecule to being able to inhibit the cancerous proliferation, passes by several stages of tests starting with the description of an activity at the cellular level. One of the strategies used for the discovery of new compounds antiproliferativs, passes by an investigation of the bioactiv potential of a great number...
Tipo: Text Palavras-chave: Microalgae; Natural products; Apoptosis; Antiproliferativ; Gene expression; Regulatory RNA; Molecular targets; Bronchopulmonary cancer; Microalgues; Molécules naturelles; Apoptose; Antiprolifératif; Expression génique; ARN régulateur; Cibles moléculaires; Cancer bronchopulmonaire.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-2196.pdf
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Etude des facteurs de transcription impliqués dans l’accumulation lipidique en condition de stress azoté chez la microalgue haptophyte Isochrysis affinis galbana ArchiMer
Thiriet-rupert, Stanislas.
In every organism, evolution and acclimation to environmental changes are orchestrated by numerous molecular players. Among them, transcription factors (TFs) play a crucial role by regulating gene expression. Therefore, identify TFs involved in the production of high value products is a significant step in a biotechnological context. The laboratory has at its disposal a mutant strain of the haptophyte microalga Tisochrysis lutea producing twice more storage lipids than the wild type strain when exposed to nitrogen deprivation. Given the key role of TFs in phenotype establishment, this PhD aim at identify the TFs involved in that of the mutant phenotype of T. lutea. A TFs identification and classification pipeline was elaborated and applied to T. lutea’s...
Tipo: Text Palavras-chave: Bioinformatique; Biologie moléculaire; Évolution; Facteur de transcription; Micro-algue; Réseau de gènes; RNA-seq; Bioinformatic; Evolution; Gene network; Molecular biology; Microalgae; RNAseq; Transcription factor.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48233/48368.pdf
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Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement ArchiMer
Geiger, Marie.
The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...
Tipo: Text Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf
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Evaluation of CO2 Biofixation and Biodiesel Production by Spirulina (Arthospira) Cultivated In Air-Lift Photobioreactor BABT
Morais,Etiele Greque de; Cassuriaga,Ana Paula Aguiar; Callejas,Nicolás; Martinez,Natalia; Vieitez,Ignacio; Jachmanián,Iván; Santos,Lucielen Oliveira; Morais,Michele Greque de; Costa,Jorge Alberto Vieira.
ABSTRACT Spirulina is a microalgal genre that has the capacity to produce various bioproducts with applications in several areas including the energy sector. The study aimed to assess the ability of CO2 biofixation, biodiesel and other biocompounds production by Spirulina sp LEB 18 cultured in air-lift photobioreactor. The microalgae presented a rich macronutrient composition: protein (47.3%), carbohydrates (13.4%) and a high lipid content (32.7%) in a media with nitrogen reduction, CO2 using air-lift photobiorector. Furthermore, 160 mg.L.d-1 of CO2 was biofixed, generating a maximum biomass yield of 0.02 g.L.d-1. The lipids evaluated for biodiesel production presented a theoretical yield of 19.8% for in situ transesterification and 47.9% for conventional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofuels; Carbon dioxide; Microalgae; Photobiorefinary; Biocompounds.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100703
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Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds ArchiMer
Réveillon, Damien; Tunin-ley, Alina; Grondin, Isabelle; Othmani, Ahlem; Zubia, Mayalen; Bunet, Robert; Turquet, Jean; Culioli, Gérald; Briand, Jean-françois.
Marine microalgae and cyanobacteria have largely been studied for their biotechnological potential and proved their ability to produce a wide array of bioactive molecules. We investigated the antifouling potential of unexplored benthic tropical microalgae using anti-adhesion and toxicity bioassays against two major micro- and ma crobiofoulers, namely bacteria and barnacles. Fifty strains belonging to six phyla [Cyanobacteria, Miozoa (Dinoflagellata), Bacillariophyta, Cryptophyta, Rhodophyta and Haptophyta] were isolated from southwestern Islands of the Indian Ocean. They were chosen in order to represent as much as possible the huge biodiversity of such a rich tropical ecosystem. The associated chemodiversity was highlighted by both NMR- and LC-MS-based...
Tipo: Text Palavras-chave: Microalgae; Antifouling; Bioassay; Chemodiversity; Bioprospecting; Metabolomics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00454/56608/58333.pdf
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Growth and biochemical composition of Chlorella vulgaris in different growth media Anais da ABC (AABC)
CHIA,MATHIAS A.; LOMBARDI,ANA T.; MELãO,MARIA DA GRAÇA G..
The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Lipid class; Microalgae; Proteins.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401427
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Growth of microalgae Scenedesmus sp in ethanol vinasse BABT
Ramirez,Nelzy Neyza Vargas; Farenzena,Marcelo; Trierweiler,Jorge Otávio.
This study evaluated the feasibility of using vinasse as a nutrient source for microalgae cultivation. The Scenedesmus sp was grown in a medium supplemented with vinasse and process variables were optimized using a factorial design and a Central Composite Design (CCD). The factorial design results showed that it was possible to cultivate microalgae at concentrations of up to 40% of vinasse in the culture medium. The CCD results showed that the light intensity and vinasse concentration influenced the amount of biomass produced.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vinasse; Microalgae; Scenedesmussp; Photobioreactor air-lift.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500630
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Harvesting Microalgal Biomass grown in Anaerobic Sewage Treatment Effluent by the Coagulation-Flocculation Method: Effect of pH BABT
Cassini,Servio Tulio; Francisco,Sara Aparecida; Antunes,Paulo Wagner Pereira; Oss,Rodrigo Nunes; Keller,Regina.
ABSTRACT Harvesting is a critical step in microalgal biomass production process for many reasons. Among the existing techniques available for harvesting and dewatering microalgal biomass, recovery from aqueous medium by coagulation-flocculation has been the most economically viable process, althoughit is highly dependent on pH. This study aims to assess alternative coagulants compared to the standard coagulant aluminum sulfate for microalgal biomass recovery from anaerobic effluent of domestic sewage treatment. The effluent quality was also analyzed after biomass recovery. Coagulants represented by modified tannin, cationic starch and aluminum sulfate recovered more than 90% of algae biomass, at concentrations greater than 80 mg/L, in the pH range 7-10....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Anaerobic effluent; Coagulation-flocculation; PH.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100700
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Importance of ecological interactions during wastewater treatment using High Rate Algal Ponds under different temperate climates ArchiMer
Galès, Amandine; Bonnafous, Anaïs; Carré, Claire; Jauzein, Vincent; Lanouguère, Elodie; Le Floc'H, Emilie; Pinoit, Joanna; Poullain, Clothilde; Roques, Cécile; Sialve, Bruno; Simier, Monique; Steyer, Jean-philippe; Fouilland, Eric.
Several studies focused on wastewater treatment in High Rate Algal Ponds (HRAP) suggest that highly variable climatic conditions cause large variations of microalgal biomass productivity. In the present study, we show that similar carbon, nitrogen and phosphorus removal efficiencies were reached in different HRAPs treating urban wastewaters located in two different temperate (Mediterranean and oceanic) climates. Furthermore, similar ecological successions were observed in these HRAPs. During the start-up phase, the consumption of organic matter by detritivores, already present in the wastewater, appears to be necessary for the microalgae to grow within two weeks in spring. The growth of the rapid-growing species, Chlorella sp., followed by the...
Tipo: Text Palavras-chave: Microalgae; Predators; Bacteria; Urban effluents; Ammonia and phosphate removal.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00490/60210/63713.pdf
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Influence de la matière organique dissoute naturelle et d’herbicides, seuls et combinés, sur des microalgues marines et d’eau douce ArchiMer
Coquillé, Nathalie.
Microalgae are at the basis of aquatic food webs and may be the indirect target of herbicides from agricultural and urban uses. They also interact with other compounds from their environment such as natural dissolved organic matter (DOM), which can itself interact with herbicides. This thesis aimed to study, in laboratory controlled conditions, the influence of natural DOM on herbicide toxicity to microalgae by using monospecific, non-axenic cultures. The experiments included: natural freshwater and marine DOM, three herbicides (irgarol, diuron and S-metolachlor) singly and in mixtures, two freshwater microalgae (Gomphonema gracile and Sphaerellopsis sp.) and two marine microalgae (Chaetoceros calcitrans and Tetraselmis suecica). Effects were evaluated on...
Tipo: Text Palavras-chave: Microalgues; Matière organique dissoute naturelle; MOD; Herbicides; Transformations chimiques; Toxicité.; Microalgae; Natural dissolved organic matter; DOM; Herbicides; Chemical transformations; Toxicity.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00461/57245/59250.pdf
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INFLUENCE OF DIFFERENT DIETS ON LENGTH AND BIOMASS PRODUCTION OF BRINE SHRIMP ARTEMIA FRANCISCANA (KELLOG, 1906)A. Boletín de Investigaciones
García-Ulloa Gómez,Manuel; Gamboa Delgado,Julián; Zavala Aguirre,José Luis; Ogura Fujii,Tetsuya; Lavens,Patrick.
Total length and biomass production of the brine shrimp Artemia franciscana were studied fed on soybean and wheat micropulverized meals (applied alone or mixed at different proportions), live microalgae (Tetraselmis suecica and Chaetoceros calcitrans), and dried Spirulina as diets. Eight diets were tested in triplicates during 10 days. Significant differences (P < 0.05) were observed from day 1 onwards. The mixed meal-based diets showed better production results. At day 1, the Artemia nauplii fed on the 70% wheat meal/ 30% soya meal diet were 30% longer compared to the animals from the C. calcitrans group. At day 10, the organisms fed with the 100% soya meal diet were 68% longer than those fed on the C. calcitrans diet. The final biomass production (wet...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia franciscana; Soya meal; Wheat meal; Microalgae; Length; Biomass production.
Ano: 1999 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97611999000100001
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Influence of nutrients on biomass and oil yield from microalgae Chlorella vulgaris for biodiesel production Rev. Ciênc. Agron.
Araujo,Glacio Souza; Lopes,Dilliani Naiane Mascena; Santiago,Clarice da Silva; Silva,José William Alves da; Fernandes,Fabiano André Narciso.
ABSTRACT Microalgae are commonly used in aquiculture as feed for postlarval mollusks, fish and crustaceans because they are easy to grow, small in size, grow rapidly and have high levels of fatty acids. These microorganisms also accumulate high amounts of oil, which can be extracted and converted into biodiesel using chemical processes. In this work, freshwater microalgae Chlorella vulgaris was grown in the Live Food Production Laboratory (LABPAV/IFCE Aracati Campus), with urea (stock solution 1), triple superphosphate (stock solution 2) and vitamins (stock solution 3), in growth medium, in triplicate, using three different quantities of stock solutions 1 and 2, but with a constant amount of vitamins. The quantities of 0.5, 1 and 2 mL (T0.5, T1 and T2,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodiesel; Microalgae; Oil.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000100404
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Interactions between polystyrene microplastics and marine phytoplankton lead to species-specific hetero-aggregation ArchiMer
Long, Marc; Paul-pont, Ika; Hegaret, Helene; Moriceau, Brivaela; Lambert, Christophe; Huvet, Arnaud; Soudant, Philippe.
To understand the fate and impacts of microplastics (MP) in the marine ecosystems, it is essential to investigate their interactions with phytoplankton as these may affect MP bioavailability to marine organisms as well as their fate in the water column. However, the behaviour of MP with marine phytoplanktonic cells remains little studied and thus unpredictable. The present study assessed the potential for phytoplankton cells to form hetero-aggregates with small micro-polystyrene (micro-PS) particles depending on microalgal species and physiological status. A prymnesiophycea, Tisochrysis lutea, a dinoflagellate, Heterocapsa triquetra, and a diatom, Chaetoceros neogracile, were exposed to micro-PS (2 μm diameter; 3.96 μg L−1) during their growth culture...
Tipo: Text Palavras-chave: Polystyrene; Microplastic; Aggregates; Microalgae; Marine; Phytoplankton.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00389/50016/50735.pdf
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Interactions microalgues-bactéries en système expérimental bispécifique : effets sur la croissance de Dunaliella sp. ArchiMer
Le Chevanton, Myriam.
The use of algae-bacteria mixed culture has been suggested to improve performance of industrial microalgae culture. The present study aims to investigate impact of selected bacteria on Dunaliella sp. growth. 97 bacteria strains were isolated from a variety of marine microalgae cultures. The diversity of strains revealed members of α-and γ-proteobacteria, Cytophaga-Flavobacterium-Bacteroides and grampositive. A two-phase approach was then implemented to select bacteria that improve Dunaliella sp. growth, using an original high-throughput experimental device. Although a majority of bacteria induced performance reduction for Dunaliella sp., some resulted in significant improvements. In nitrogenlimited batch culture, the Alteromonas sp. bacteria demonstrated a...
Tipo: Text Palavras-chave: Interactions; Microalgues; Bactéries; Dunaliella; Axénie; Interactions; Microalgae; Bacteria; Dunaliella; Axenic.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00187/29839/28291.pdf
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Les besoins écophysiologiques des larves d'huitres creuse Crassostrea gigas en conditions contrôlees : effet de la température, de la nourriture et modélisation de la croissance ArchiMer
Rico Villa, Benjamin.
Nowadays, spat production of Pacific oyster Crassostrea gigas relies on rearing techniques under controlled conditions in hatchery. In this context, an extensive research in aquaculture is necessary because larval development and metamorphosis are critical for hatchery management. Firstly, a diet consisted in a Haptophyceae: Isochrysis affinis galbana (clone T-ISO) and a Bacillariophyceae: Chaetoceros calcitrans forma pumilum demonstrated its efficiency to supply the nutritional needs throughout the whole larval rearing. Then, a flow-through rearing system was developed and coupled with a continuous hydrobiological data recorder to provide basic information for ecophysiology research through different trophic levels and/or temperature throughout larval...
Tipo: Text Palavras-chave: Crassostrea gigas; Larve; Huître; Croissance; Ecophysiologie; Microalgues; Température; Système d’élevage; Modélisation; Crassostrea gigas; Larvae; Oyster; Growth; Ecophysiology; Microalgae; Temperature; Rearing System; Modelling.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00012/12308/9102.pdf
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